Overview
Type: Synthetic peptide
Form: Lyophilized powder
NCX2/SLC8A2 Blocking Peptide (#BLP-NX012) is the original antigen used for immunization during Anti-NCX2 (SLC8A2) Antibody (#ANX-012) generation. The blocking peptide binds and ‘blocks’ Anti-NCX2/SLC8A2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat skeletal muscle lysate (lanes 3 and 7) and human SH-SY5Y neuroblastoma cell lysate (lanes 4 and 8):1-4. Anti-NCX2 (SLC8A2) Antibody (#ANX-012), (1:200).
5-8. Anti-NCX2 (SLC8A2) Antibody, preincubated with NCX2/SLC8A2 Blocking Peptide (#BLP-NX012).
Expression of NCX2 in rat cerebellumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NCX2 (SLC8A2) Antibody (#ANX-012), (1:100). A. NCX2 staining (red) is expressed in the molecular layer (M) and Purkinje cells (arrows). B. DAPI counterstain (blue) displays the layout of cerebellar layers. C. Merge of A and B demonstrates restriction of NCX2 expression to the molecular layer and Purkinje cells.
Properties
Sequence
- (C)RVGDAQGMFEPDGG, corresponding to amino acid residues 486-499 of rat NCX2 (Accession P48768).
Accession (Uniprot) Number P48768
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
