Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)RVGDAQGMFEPDGG, corresponding to amino acid residues 486-499 of rat NCX2 (Accession P48768).
Accession (Uniprot) Number P48768
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat skeletal muscle lysate (lanes 3 and 7) and human SH-SY5Y neuroblastoma cell lysate (lanes 4 and 8):1-4. Anti-NCX2 (SLC8A2) Antibody (#ANX-012), (1:200).
5-8. Anti-NCX2 (SLC8A2) Antibody, preincubated with NCX2/SLC8A2 Blocking Peptide (#BLP-NX012).- Rat brain frozen sections (immersion-fixed, free floating), (1:100).
Last update: 02/01/2024
