Overview
Type: Synthetic peptide
Form: Lyophilized powder
NCX3/SLC8A3 Blocking Peptide (#BLP-NX013) is the original antigen used for immunization during Anti-NCX3 (SLC8A3) Antibody (#ANX-013) generation. The blocking peptide binds and ‘blocks’ Anti-NCX3/SLC8A3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates (lanes 1 and 4), mouse brain lysates (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6):1-3. Anti-NCX3 (SLC8A3) Antibody (#ANX-013), (1:200).
4-6. Anti-NCX3 (SLC8A3) Antibody, preincubated with NCX3/SLC8A3 Blocking Peptide (#BLP-NX013).
Expression of NCX3 in rat cerebellumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NCX3 (SLC8A3) Antibody (#ANX-013), (1:100). A. NCX3 staining (green) is expressed mostly in molecular layer (Mol) interneurons (arrow). B. The same section, stained with Parvalbumin (Red). C. Merge of A and B demonstrates that NCX3 appears to be expressed in several GABAergic neurons in the molecular layer of which Parvalbumin positive cells are only one sub-group (see arrow). DAPI counterstain (blue) displays the layout of cerebellar layers.
Properties
Sequence
- (C)PLEGKEVDESRRE, corresponding to amino acid residues 303-315 of rat NCX3 (Accession P70549).
Accession (Uniprot) Number P70549
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
