Cat #: BLP-NX013
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)PLEGKEVDESRRE, corresponding to amino acid residues 303-315 of rat NCX3 (Accession P70549).
Accession (Uniprot) Number P70549
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain lysates (lanes 1 and 4), mouse brain lysates (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6):1-3. Anti-NCX3 (SLC8A3) Antibody (#ANX-013), (1:200).
4-6. Anti-NCX3 (SLC8A3) Antibody, preincubated with NCX3/SLC8A3 Blocking Peptide (#BLP-NX013). - Rat immersion-fixed, free floating brain sections (1:100).
Last update: 02/01/2024