Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nectin-1/PVRL1 (extracellular) Blocking Peptide (#BLP-NR051) is the original antigen used for immunization during Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051) generation. The blocking peptide binds and ‘blocks’ Anti-Nectin-1/PVRL1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051), (1:200).
3,4. Anti-Nectin-1/PVRL1 (extracellular) Antibody, preincubated with Nectin-1/PVRL1 (extracellular) Blocking Peptide (#BLP-NR051).
Western blot analysis of human Jurkat T-cell leukemia (lanes 1 and 3) and human MEG-01 chronic myelogenous leukemia (lanes 2 and 4) cell lysates:1,2. Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051), (1:200).
3,4. Anti-Nectin-1/PVRL1 (extracellular) Antibody, preincubated with Nectin-1/PVRL1 (extracellular) Blocking Peptide (#BLP-NR051).
Expression of Nectin-1 in rat cerebellumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051), (1:100). A. Nectin-1 staining (red) is apparent in Purkinje neurons and their dendritic tree (arrow). B. Cell nuclei in the same section are visualized with DAPI (blue). C. Merge of the two images.
Properties
Sequence
- (C)GKPPSVVSWETRLK, corresponding to amino acid residues 177-190 of human Nectin-1 (Accession Q15223).
Accession (Uniprot) Number Q15223
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
