Overview
Type: Synthetic peptide
Form: Lyophilized Powder
NEGR1 (extracellular) Blocking Peptide (#BLP-NR058) is the original antigen used for immunization during Anti-NEGR1 (extracellular) Antibody (#ANR-058) generation. The blocking peptide binds and ‘blocks’ Anti-NEGR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain synaptosomes (lanes 2 and 4):1-2. Anti-NEGR1 (extracellular) Antibody (#ANR-058), (1:600).
3-4. Anti-NEGR1 (extracellular) Antibody, preincubated with NEGR1 (extracellular) Blocking Peptide (BLP-NR058).
Western blot analysis of mouse pancreas lysates (lanes 1 and 3) and mouse colon lysates (lanes 2 and 4):1-2. Anti-NEGR1 (extracellular) Antibody (#ANR-058), (1:400).
3-4. Anti-NEGR1 (extracellular) Antibody, preincubated with NEGR1 (extracellular) Blocking Peptide (BLP-NR058).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4), human Caco-2 colon adenocarcinoma cell line lysate (lanes 2 and 5) and human HT-29 colorectal adenocarcinoma cell line lysate (lanes 3 and 6):1-3. Anti-NEGR1 (extracellular) Antibody (#ANR-058), (1:400).
4-6. Anti-NEGR1 (extracellular) Antibody, preincubated with NEGR1 (extracellular) Blocking Peptide (BLP-NR058).
Expression of NEGR1 in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NEGR1 (extracellular) Antibody (#ANR-058), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NEGR1 immunoreactivity (green) appears in neuron outlines (arrows). B. Pre-incubation of the antibody with NEGR1 (extracellular) Blocking Peptide (BLP-NR058), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)HISPSAKPFENGQYLD, corresponding to amino acid residues 168 -183 of mouse NEGR1 (Accession Q80Z24). Extracellular.
Accession (Uniprot) Number Q80Z24
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
