Overview
Type: Synthetic peptide
Form: Lyophilized powder
NETO1 (extracellular) Blocking Peptide (#BLP-GC045) is the original antigen used for immunization during Anti-NETO1 (extracellular) Antibody (#AGC-045) generation. The blocking peptide binds and ‘blocks’ Anti-NETO1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2 Anti-NETO1 (extracellular) Antibody (#AGC-045), (1:200).
3,4. Anti-NETO1 (extracellular) Antibody, preincubated with NETO1 (extracellular) Blocking Peptide (#BLP-GC045).
Expression of NETO1 in rat and mouse cortex.Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-NETO1 (extracellular) Antibody (#AGC-045), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. NETO1 staining in rat (A) and mouse (B) sections (green), is detected in cells in the deep pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KKGTEKQITPETQK, corresponding to amino acid residues 24-37 of mouse NETO1 (Accession Q8R4I7).
Accession (Uniprot) Number Q8R4I7
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
