Cat #: BLP-GC046
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)RSSQVEQEEKTKPG, corresponding to amino acid residues 189-202 of rat NETO2 (Accession C6K2K4).
Accession (Uniprot) Number C6K2K4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-NETO2 (extracellular) Antibody (#AGC-046), (1:200).
3,4. Anti-NETO2 (extracellular) Antibody, preincubated with NETO2 (extracellular) Blocking Peptide (#BLP-GC046). - Western blot analysis of human SH-SY5Y neuroblastoma (lanes 1 and 4), human HT-29 colon adenocarcinoma (lanes 2 and 5) and human MCF-7 breast adenocarcinoma (lanes 3 and 6) cell line lysates:1-3. Anti-NETO2 (extracellular) Antibody (#AGC-046), (1:200).
4-6. Anti-NETO2 (extracellular) Antibody, preincubated with NETO2 (extracellular) Blocking Peptide (#BLP-GC046). - Mouse and rat brain sections (1:200).
Last update: 02/01/2024