Overview
Type: Synthetic peptide
Form: Lyophilized powder
Neurokinin 1 Receptor/NK1R (extracellular) Blocking Peptide (#BLP-TR001) is the original antigen used for immunization during Anti-Neurokinin 1 Receptor (NK1R) (extracellular) Antibody (#ATR-001) generation. The blocking peptide binds and ‘blocks’ Anti-Neurokinin 1 Receptor/NK1R (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat ovary (lanes 1 and 5), rat brain (lanes 2 and 6), mouse brain membrane (lanes 3 and 7) and human SH-SY5Y brain neuroblastoma (lanes 4 and 8) lysate:1-4. Anti-Neurokinin 1 Receptor (NK1R) (extracellular) Antibody (#ATR-001), (1:200).
5-8. Anti-Neurokinin 1 Receptor (NK1R) (extracellular) Antibody, preincubated with Neurokinin 1 Receptor/NK1R (extracellular) Blocking Peptide (#BLP-TR001).
Expression of Neurokinin 1 receptor in rat colonImmunohistochemical staining of rat colon paraffin-embedded section using Anti-Neurokinin 1 Receptor (NK1R) (extracellular) Antibody (#ATR-001), (1:100) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. A. NK1 receptor labeling appears in the tubular glands of the mucosa layer. Note that the smooth muscle and lamina propria do not stain. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
Properties
Sequence
- CMIEWPEHPNRTYEK, corresponding to amino acid residues 180-194 of rat NK1 receptor (Accession P14600).
Accession (Uniprot) Number P14600
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 mg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
