Overview
Type: Synthetic peptide
Form: Lyophilized powder
Neurokinin 2 Receptor/NK2R Blocking Peptide (#BLP-TR002) is the original antigen used for immunization during Anti-Neurokinin 2 Receptor (NK2R) Antibody (#ATR-002) generation. The blocking peptide binds and ‘blocks’ Anti-Neurokinin 2 Receptor/NK2R primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat hippocampus (lanes 1 and 3) and rat uterus (lanes 2 and 4):1,2. Anti-Neurokinin 2 Receptor (NK2R) Antibody (#ATR-002), (1:200).
3,4. Anti-Neurokinin 2 Receptor (NK2R) Antibody, preincubated with Neurokinin 2 Receptor/NK2R Blocking Peptide (#BLP-TR002).Rat nodose ganglion sections (Yuan, M. et al. (2019) CNS Neurosci. Ther. 25, 123.).
Properties
Sequence
- CHTKETLFMTGDMTHSE, corresponding to amino acid residues 351-367 of rat NK2 receptor (Accession P16610).
Accession (Uniprot) Number P16610
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
