Overview
Type: Synthetic peptide
Form: Lyophilized powder
Neuroligin 1 (extracellular) Blocking Peptide (#BLP-NR035) is the original antigen used for immunization during Anti-Neuroligin 1 (extracellular) Antibody (#ANR-035) generation. The blocking peptide binds and ‘blocks’ Anti-Neuroligin 1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Neuroligin 1 (extracellular) Antibody (#ANR-035), (1:800).
3,4. Anti-Neuroligin 1 (extracellular) Antibody preincubated with Neuroligin 1 (extracellular) Blocking Peptide (#BLP-NR035).
Expression of NLGN1 in rat DRGImmunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-Neuroligin 1 (extracellular) Antibody (#ANR-035) followed by anti-rabbit-AlexaFluor-594 secondary antibody. A. NLGN1 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.
Expression of NLGN1 in rat hippocampus and cortexImmunohistochemical staining of free floating frozen section of rat hippocampal CA1 region (A, B) and rat parietal cortex (C, D) using Anti-Neuroligin 1 (extracellular) Antibody (#ANR-035), (1:300). A. NLGN1 staining (green) appears in several neuronal profiles in the pyramidal layer (arrows). C. NLGN1 staining (green) is detected in several neuronal profiles including pyramidal cells and their apical dendrite (arrows). B, D. Nuclei are stained using DAPI as the counterstain (blue).
Properties
Sequence
- (C)KVPSTDITLRPTRK, corresponding to amino acid residues 648-661 of rat NLGN1 (Accession Q62765).
Accession (Uniprot) Number Q62765
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
