Overview
Type: Synthetic peptide
Form: Lyophilized powder
Neuroligin 3 (extracellular) Blocking Peptide (#BLP-NR037) is the original antigen used for immunization during Anti-Neuroligin 3 (extracellular) Antibody (#ANR-037) generation. The blocking peptide binds and ‘blocks’ Anti-Neuroligin 3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate:1,2. Anti-Neuroligin 3 (extracellular) Antibody (#ANR-037), (1:200).
3,4. Anti-Neuroligin 3 (extracellular) Antibody, preincubated with Neuroligin 3 (extracellular) Blocking Peptide (#BLP-NR037).
Expression of Neuroligin-3 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuroligin 3 (extracellular) Antibody (#ANR-037), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Neuroligin-3 immunoreactivity (green) appears in in the granule layer (GL) and in some purkinje cells (arrows). B. Pre-incubation of the antibody with Neuroligin 3 (extracellular) Blocking Peptide (BLP-NR037), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Neuroligin-3 in rat deep cerebellar nuclei.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuroligin 3 (extracellular) Antibody (#ANR-037), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Neuroligin-3 immunoreactivity (green) appears in deep cerebellar nuclei forming neuronal outlines (arrows). B. Pre-incubation of the antibody with Neuroligin 3 (extracellular) Blocking Peptide (BLP-NR037), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)SHITRRPNGKTWSTKR, corresponding to amino acid residues 656-671 of rat NLGN3 (Accession Q62889).
Accession (Uniprot) Number Q62889
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
