Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (#BLP-NR191) is the original antigen used for immunization during Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191) generation. The blocking peptide binds and ‘blocks’ Anti-Neuronal Pentraxin 1 (NPTX1) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191), (1:400).
3-4. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody, preincubated with Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (BLP-NR191).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):1-2. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191), (1:400).
3-4. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody, preincubated with Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (BLP-NR191).
Expression of Neuronal Pentraxin 1 in rat striatum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NPTX1 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (BLP-NR191), suppressed staining. Cell nuclei are stained with DAPI (blue). CC = corpus callosum, ST = striatum.
Properties
Sequence
- Peptide (C)ERAKIESALTSLHQR, corresponding to amino acid residues 197 - 211 of mouse NPTX1 (Accession Q62443).
Accession (Uniprot) Number Q62443
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
