Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Neuropeptide FF Receptor 1 (extracellular) Blocking Peptide (#BLP-NR073) is the original antigen used for immunization during Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody (#ANR-073) generation. The blocking peptide binds and ‘blocks’ Anti-Neuropeptide FF Receptor 1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody (#ANR-073), (1:200).
3-4. Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody, preincubated with Neuropeptide FF Receptor 1 (extracellular) Blocking Peptide (BLP-NR073).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioma cell line lysate (lanes 2 and 4):1-2. Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody (#ANR-073), (1:200).
3-4. Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody, preincubated with Neuropeptide FF Receptor 1 (extracellular) Blocking Peptide (BLP-NR073).
Expression of NPFFR1 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody (#ANR-073), (1:400), followed by goat anti-rabbit-AlexaFluor-488. A. NPFFR1 immunoreactivity (green) appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with Neuropeptide FF Receptor 1 (extracellular) Blocking Peptide (BLP-NR073), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of NPFFR1 in rat hypothalamusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody (#ANR-073), (1:400), followed by goat anti-rabbit-AlexaFluor-488. A. NPFFR1 immunoreactivity (green) appears in neuronal outlines (arrows) in the ventromedial hypothalamus. B. Pre-incubation of the antibody with Neuropeptide FF Receptor 1 (extracellular) Blocking Peptide (BLP-NR073), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third ventricle
Properties
Sequence
- Peptide (C)REEHHFMLDARNRS, corresponding to amino acid residues 184 - 197 of rat NPFFR1 (Accession Q9EP86).
Accession (Uniprot) Number Q9EP86
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
