Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Neuropeptide FF Receptor 2 (extracellular) Blocking Peptide (#BLP-NR074) is the original antigen used for immunization during Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (#ANR-074) generation. The blocking peptide binds and ‘blocks’ Anti-Neuropeptide FF Receptor 2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and rat dorsal root ganglion lysate (lanes 2 and 4):1-2. Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (#ANR-074), (1:200).
3-4. Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody, preincubated with Neuropeptide FF Receptor 2 (extracellular) Blocking Peptide (BLP-NR074).
Western blot analysis of mouse Neuro-2a neuroblastoma cell line lysate (lanes 1 and 4), human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):1-3. Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (#ANR-074), (1:200).
4-6. Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody, preincubated with Neuropeptide FF Receptor 2 (extracellular) Blocking Peptide (BLP-NR074).
Properties
Sequence
- Peptide ((C)EEKYYRVRLSSHNK, corresponding to amino acid residues 186 - 199 of mouse NPFFR2 (Accession Q924H0). Extracellular, 2nd loop.
Accession (Uniprot) Number Q924H0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
