Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Neuropeptide S Receptor (extracellular) Blocking Peptide (#BLP-NR029) is the original antigen used for immunization during Anti-Neuropeptide S Receptor (extracellular) Antibody (#ANR-029) generation. The blocking peptide binds and ‘blocks’ Anti-Neuropeptide S Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 5), rat brain lysate (lanes 2 and 6), rat small intestine lysate (lanes 3 and 7) and rat lung lysate (lanes 4 and 8):1-4. Anti-Neuropeptide S Receptor (extracellular) Antibody (#ANR-029), (1:200).
5-8. Anti-Neuropeptide S Receptor (extracellular) Antibody, preincubated with Neuropeptide S Receptor (extracellular) Blocking Peptide (BLP-NR029).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate:1. Anti-Neuropeptide S Receptor (extracellular) Antibody (#ANR-029), (1:200).
2. Anti-Neuropeptide S Receptor (extracellular) Antibody, preincubated with Neuropeptide S Receptor (extracellular) Blocking Peptide (BLP-NR029).
Expression of Neuropeptide S Receptor in rat supraoptic nucleus (SON).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuropeptide S Receptor (extracellular) Antibody (#ANR-029), (1:400), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. NPSR1 immunoreactivity (red) appears in SON neurons (arrows). B. Pre-incubation of the antibody with Neuropeptide S Receptor (extracellular) Blocking Peptide (BLP-NR029), suppressed staining. Cell nuclei are stained with DAPI (blue). OT = optic tract, SON = supraoptic nucleus.
Properties
Sequence
- Peptide (C)DNFNVLPDTKERFYAS, corresponding to amino acid residues 297 - 312 of mouse NPSR1 (Accession Q8BZP8).
Accession (Uniprot) Number Q8BZP8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
