Overview
Type: Synthetic peptide
Form: Lyophilized powder
Neuropilin-2/NRP2 (extracellular) Blocking Peptide (#BLP-NR062) is the original antigen used for immunization during Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062) generation. The blocking peptide binds and ‘blocks’ Anti-Neuropilin-2/NRP2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4):1,2. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062), (1:200).
3,4. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody, preincubated with Neuropilin-2/NRP2 (extracellular) Blocking Peptide (#BLP-NR062).
Western blot analysis of human U-87 MG glioma cell line lysate:1. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062), (1:200).
2. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody, preincubated with Neuropilin-2/NRP2 (extracellular) Blocking Peptide (#BLP-NR062).
Expression of Neuropilin-2 in mouse substantia nigra pars compactaImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062), (1:400). A. NRP2 staining (green) reveals several neuronal profiles of NRP2 positive neurons that are distributed along the substantia nigra pars compacta. B. The same section stained for Calbindin-D28K (red) shows positive neurons along the substantia nigra pars compacta. C. Merge of the two images reveals co-localization in several neurons (arrows). DAPI counterstain is used to visualize nuclei of all cells (blue).
Properties
Sequence
- (C)REDQGGEWKHGR, corresponding to amino acid residues 747-758 of human neuropilin-2 (Accession O60462).
Accession (Uniprot) Number O60462
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
