Overview
Type: Synthetic peptide
Form: Lyophilized powder
Neuroplastin (extracellular) Blocking Peptide (#BLP-NR090) is the original antigen used for immunization during Anti-Neuroplastin (extracellular) Antibody (#ANR-090) generation. The blocking peptide binds and ‘blocks’ Anti-Neuroplastin (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 4), rat cerebellum lysate (lanes 2 and 5) and mouse brain membrane (lanes 3 and 6):1-3. Anti-Neuroplastin (extracellular) Antibody (#ANR-090), (1:400).
4-6. Anti-Neuroplastin (extracellular) Antibody, preincubated with Neuroplastin (extracellular) Blocking Peptide (#BLP-NR090).
Expression of Neuroplastin in rat hippocampus and rat cortexImmunohistochemical staining of free floating frozen section of rat hippocampal CA1 region (A) and posterior cingulate cortex (B) using Anti-Neuroplastin (extracellular) Antibody (#ANR-090), (1:120). A. NPTN staining (green) appears in neurons (vertical arrows) of the pyramidal layer (P) and in apical dendrites (horizontal arrows). B. NPTN staining (green) is detected in pyramidal neurons in the soma (vertical arrows) and in their apical dendrites (diagonal arrows). Nuclei were stained using DAPI as the counterstain (blue).
Properties
Sequence
- (C)RKNASNMEYRINKPRAE, corresponding to amino acid residues 194-210 of rat NPTN (Accession P97546).
Accession (Uniprot) Number P97546
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
