Overview
Type: Synthetic peptide
Form: Lyophilized powder
Neurotensin Receptor 1 (extracellular) Blocking Peptide (#BLP-NT015) is the original antigen used for immunization during Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015) generation. The blocking peptide binds and ‘blocks’ Anti-Neurotensin Receptor 1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human colon cancer HT-29 (lanes 1 and 4), human lung small cell carcinoma NCI-H526 (lanes 2 and 5) and human breast adenocarcinoma MDA-MB-468 (lanes 3 and 6) cell lysates:1-3. Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015), (1:200).
4-6. Anti-Neurotensin Receptor 1 (extracellular) Antibody, preincubated with Neurotensin Receptor 1 (extracellular) Blocking Peptide (#BLP-NT015).
Expression of NTSR1 in human breast cancerImmunohistochemical staining of human breast cancer cells using Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015), (1:100). Staining (brown color) is specific for epithelium-derivated malignant cells. Hematoxilin is used as the counterstain.
Expression of NTSR1 in rat brainImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015), (1:100). A. NTSR1 staining (green) appears in cells of the diagonal band region. B. The same section was stained with Choline acetyltransferase (ChAT) (red), a marker of cholinergic neurons. C. Merge of the two images demonstrates expression of NTSR1 in cholinergic neurons. Arrows point at examples of colocalization. Cell nuclei were stained with DAPI (blue).
Properties
Sequence
- EQNRSADGQHAGGLVC corresponding to amino acid residues 209-224 of human NTSR1 (Accession P30989).
Accession (Uniprot) Number P30989
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
