Cat #: BLP-NT015
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- EQNRSADGQHAGGLVC corresponding to amino acid residues 209-224 of human NTSR1 (Accession P30989).
Accession (Uniprot) Number P30989
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of human colon cancer HT-29 (lanes 1 and 4), human lung small cell carcinoma NCI-H526 (lanes 2 and 5) and human breast adenocarcinoma MDA-MB-468 (lanes 3 and 6) cell lysates:1-3. Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015), (1:200).
4-6. Anti-Neurotensin Receptor 1 (extracellular) Antibody, preincubated with Neurotensin Receptor 1 (extracellular) Blocking Peptide (#BLP-NT015). - Human breast cancer sections (paraffin embedded) and rat brain sections (1:100).
Last update: 02/01/2024