Certificate of Analysis

Neurotensin

Name: Neurotensin
Brand: Alomone Labs
SKU: GPN-100

NTS, NTS1, NT

A Ligand of Neurotensin G-Protein Coupled Receptor

Back to product page SDS

Overview

Cat #: GPN-100

Alternative Name NTS, NTS1, NT

Lyophilized Powder yes

Origin Synthetic peptide

MW: 1689.99 Da

Purity: >98% (HPLC)

Effective concentration 5-200 nM.

Sequence ELYENKPRRPYIL.

Modifications pyro-Glu¹.

Molecular formula C₇₈H₁₂₁N₂₁O₂₀.

CAS No.: 55508-42-4

Activity Neurotensin is an endocrinic or paracrinic hormone. It plays a role in the regulation of fat metabolism, feeding suppression, circadian pacemaker, anti-psychotic-like action, body temperature, dopamine neurotransmission, and smooth muscle contraction and vasodilatation^1,2.

References-Activity

Carraway, R., Leeman, S. E. (1973) J. Biol. Chem. 248, 6854.
Turner, J.T. et al. (1990) J. Pharmacol. Exp. Ther. 253, 1049.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions Up to one week at 4°C or three months at -20°C.

Our bioassay

Alomone Labs Neurotensin induces Ca²⁺ influx in HT29 cells.
Cells were loaded with Fluo-3AM Ca²⁺ probe. Ca²⁺ influx was measured in the presence of increasing Neurotensin (#GPN-100) concentrations. The maximum Ca²⁺ levels is plotted for each neurotensin concentration (ED₅₀= 142 ng/ml).

Scientific background

Neurotensin is a peptide neurotransmitter hormone produced from a 170 amino acid precursor protein, first isolated in 1973 from bovine hypothalamus¹. Neurotensin signaling is transmitted through three receptors, NTS1 and NTS2 are 7-transmembrane G-protein coupled receptors, and NTS3 is a type I membrane receptor with a large extra cellular domain².

Neurotensin synaptic transmission is terminated primarily by specific enzymatic cleavage of neurotensin by several peptidases³. Neurotensin participates in the regulation of Hypophyseal Prolactin and Luteinizing Hormone (LH) secretion⁴ and has a pivotal role in the dopaminergic system regulation.

Neurotensin is implicated in body temperature regulation, feeding behavior, locomotion regulation⁵ and pain transmission⁶.

GPCR ligands are now part of our growing portfolio of GPCR related products, which also includes antibodies.

Target Neurotensin Receptors

Peptide Content: 100%

Lyophilized Powder

For research purposes only, not for human use

Last Update: 07/05/2024

Specifications

Citations

Applications

Scientific Background

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

Reagent Rewards

Not sure whether the product fits your experimental setup?

Try it first!

Alomone Guarantee

We offer 100% guarantee on all our products. If our products do not perform as stated, please contact our technical support staff.

Alomone Guarantee information

Navigation

Order Summary