Cat #: BLP-NT003
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- GEIKTGNSPV(C), corresponding to amino acid residues 39-48 of mature human NT-3 (residues 177-186 of the NT-3 precursor) (Accession P20783).
Accession (Uniprot) Number P20783
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 0.5 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (100 ng):1. Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003), (1:200)2. Anti-Neurotrophin 3 (NT-3) Antibody, preincubated with Neurotrophin 3/NT-3 Blocking Peptide (#BLP-NT003).
- Mouse spinal cord. Rat hippocampus (1:300) (Ramos Languren, L.E. and Escobar, M.L. (2013) Eur. J. Neurosci. 37, 1248).
Last update: 02/01/2024