Overview
Type: Synthetic peptide
Form: Lyophilized powder
Neurotrophin 4/NT-4 Blocking Peptide (#BLP-NT004) is the original antigen used for immunization during Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004) generation. The blocking peptide binds and ‘blocks’ Anti-Neurotrophin 4/NT-4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of Recombinant human Neurotrophin-4 (NT-4) protein (#N-270), (10 ng):1. Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004), (1:200).
2. Anti-Neurotrophin 4 (NT-4) Antibody, preincubated with Neurotrophin 4/NT-4 Blocking Peptide (#BLP-NT004).
Expression of Neurotrophin 4 in mouse spinal cordImmunohistochemical staining of mouse spinal cord using Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004). The spinal ventral horn (VH) was very lightly stained. However, in the perimeter of the white matter (WM) there were NT-4 immunoreactive cells with a small soma (triangles) and some had a stained process (arrows).
Properties
Sequence
- LGEVPAAGGSPL(C), corresponding to amino acid residues 43-52 of mature human NT-4 (residues 123-132 of the precursor NT-4) (Accession P34130).
Accession (Uniprot) Number P34130
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 0.5 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
