Cat #: BLP-NT004
Size: 20 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- LGEVPAAGGSPL(C), corresponding to amino acid residues 43-52 of mature human NT-4 (residues 123-132 of the precursor NT-4) (Accession P34130).
Accession (Uniprot) Number P34130
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 0.5 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of Recombinant human Neurotrophin-4 (NT-4) protein (#N-270), (10 ng):1. Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004), (1:200).
2. Anti-Neurotrophin 4 (NT-4) Antibody, preincubated with Neurotrophin 4/NT-4 Blocking Peptide (#BLP-NT004). - Mouse spinal cord.
Last update: 02/01/2024