Overview
Type: Natural protein
Form: Lyophilized powder
NGF Blocking Peptide (#BLP-N240) is the original antigen used for immunization during Anti-NGF Antibody (#AN-240) generation. The blocking peptide binds and ‘blocks’ Anti-NGF primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis using Anti-NGF Antibody (#AN-240) (1:200).
Expression of NGF in rat brainImmunohistochemical staining of rat hippocampal mossy fiber terminal zone using Anti-NGF Antibody (#AN-240). A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity.
Properties
Sequence
- Purified NGF 2.5S
Accession (Uniprot) Number P01139
Peptide Confirmation Confirmed by DNA sequence and SDSPAGE.
Purity >95% (HPLC)
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 μl PBS.
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
