Overview
Type: Synthetic peptide
Form: Lyophilized Powder
NGL-2/LRRC4 (extracellular) Blocking Peptide (#BLP-NR162) is the original antigen used for immunization during Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162) generation. The blocking peptide binds and ‘blocks’ Anti-NGL-2/LRRC4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain synaptosome preparation (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200).
3-4. Anti-NGL-2/LRRC4 (extracellular) Antibody, preincubated with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysates:1. Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200).
2. Anti-NGL-2/LRRC4 (extracellular) Antibody, preincubated with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162).
Expression of NGL-2/LRRC4 in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. NGL-2/LRRC4 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third cerebral ventricle
Expression of NGL-2/LRRC4 in mouse cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. NGL-2/LRRC4 immunoreactivity (green) appears in cortical neurons (arrows). B. Pre-incubation of the antibody with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)STDTTDKMQTSLDE, corresponding to amino acid residues 507 - 520 of mouse LRRC4 (Accession Q99PH1).
Accession (Uniprot) Number Q99PH1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
