Overview
Type: Synthetic peptide
Form: Lyophilized powder
NGL-3/LRRC4B (extracellular) Blocking Peptide (#BLP-NR163) is the original antigen used for immunization during Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163) generation. The blocking peptide binds and ‘blocks’ Anti-NGL-3/LRRC4B (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat new born brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1,2. Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200).
3,4. Anti-NGL-3/LRRC4B (extracellular) Antibody, preincubated with NGL-3/LRRC4B (extracellular) Blocking Peptide (#BLP-NR163).
Western blot analysis of mouse brain lysate:1. Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200).
2. Anti-NGL-3/LRRC4B (extracellular) Antibody, preincubated with NGL-3/LRRC4B (extracellular) Blocking Peptide (#BLP-NR163).
Expression of NGL-3 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200), followed by goat anti-rabbit-AlexaFluor-488. NGL-3 immunoreactivity (green) is detected in the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)PGEEAQQPRGTEKE, corresponding to amino acid residues 498-511 of mouse NGL-3 (Accession P0C192).
Accession (Uniprot) Number P0C192
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
