Overview
Type: Synthetic peptide
Form: Lyophilized powder
NHERF-1/EBP50 Blocking Peptide (#BLP-PZ006) is the original antigen used for immunization during Anti-NHERF-1/EBP50 Antibody (#APZ-006) generation. The blocking peptide binds and ‘blocks’ Anti-NHERF-1/EBP50 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blotting of rat kidney membranes:1. Anti-NHERF-1/EBP50 Antibody (APZ-006), (1:200).
2. Anti-NHERF-1/EBP50 Antibody, preincubated with NHERF-1/EBP50 Blocking Peptide (#BLP-PZ006).
Properties
Sequence
- (C)IQKENSREALVEPA, corresponding to residues 261-274 of rat NHERF-1 (Accession Q9JJ19).
Accession (Uniprot) Number Q9JJ19
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
