Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nicotinic Acetylcholine Receptor α3/CHRNA3 (extracellular) Blocking Peptide (#BLP-NC003) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody (#ANC-003) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α3/CHRNA3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody (#ANC-003), (1:200).
2. Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α3/CHRNA3 (extracellular) Blocking Peptide (#BLP-NC003).
Expression of Nicotinic acetylcholine receptor α3 in rat neocortexImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections were stained using Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody (#ANC-003), (1:200). A. Intense staining (green) of nAChR α3 appears in apical dendrites (thin arrows). A few cortical interneurons (thick arrow) are also stained with the antibody. B. The same section was stained also for parvalbumin (red staining). An arrow depicts a neuron expressing both nAChR α3 and parvalbumin.
Properties
Sequence
- (C)KWKPSDYQGVEFMR, corresponding to amino acid residues 91-104 of rat nAChRα3 (Accession P04757).
Accession (Uniprot) Number P04757
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
