Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nicotinic Acetylcholine Receptor α7/CHRNA7 (extracellular) Blocking Peptide (#BLP-NC007) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α7/CHRNA7 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates:1,2. Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007), (1:200).
3,4. Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α7/CHRNA7 (extracellular) Blocking Peptide (#BLP-NC007).
Expression of nAChRα7 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007), (1:200). nAChRα7 (red staining) is expressed in DRG neurons. Hoechst 33342 is used as the counterstain (blue).
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Guinea Pig Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007-GP), (1:500).
3-4. Guinea Pig Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007-GP), preincubated with Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Blocking Peptide (#BLP-NC-007).
Expression of nAChRα7 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007-GP), (1:300), followed by goat anti-guinea pig-Alexa-594. A. Staining in the hippocampal CA1 region, showed nAChRα7 immunoreactivity (red) in the pyramidal layer (P). B. Pre-incubation of the antibody with Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Blocking Peptide (#BLP-NC-007), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of nAChRα7 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007-GP), (1:300), followed by goat anti-guinea pig-Alexa-594. A. nAChRα7 immunoreactivity (red) appears in the purkinje layer (P) and in the molecular layer (M). B. Pre-incubation of the antibody with Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Blocking Peptide (#BLP-NC-007), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of nAChRα7 in in rat substantia nigra pars compacta (SNC).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007-GP), (1:300), followed by goat anti-guinea pig-Alexa-594. A. nAChRα7 immunoreactivity (red) appears in cells of the SNC (arrows). B. Pre-incubation of the antibody with Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Blocking Peptide (#BLP-NC-007), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KELVKNYNPLER, corresponding to amino acid residues 31-42 of rat nAChRα7 (Accession Q05941).
Accession (Uniprot) Number Q05941
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
