Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nicotinic Acetylcholine Receptor β3/CHRNB3 (extracellular) Blocking Peptide (#BLP-NC013) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody (#ANC-013) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor β3/CHRNB3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody (#ANC-013), (1:400).
2. Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor β3/CHRNB3 (extracellular) Blocking Peptide (#BLP-NC013).
Properties
Sequence
- (C)NAKGMKGNRREG, corresponding to amino acid residues 211-222 of rat nAChRβ3 (Accession P12391).
Accession (Uniprot) Number P12391
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
