Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nicotinic Acetylcholine Receptor α10/CHRNA10 Blocking Peptide (#BLP-NC010) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α10 (CHRNA10) Antibody (#ANC-010) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α10/CHRNA10 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates:1. Anti-Nicotinic Acetylcholine Receptor α10 (CHRNA10) Antibody (#ANC-010), (1:500).
2. Anti-Nicotinic Acetylcholine Receptor α10 (CHRNA10) Antibody, preincubated with Nicotinic Acetylcholine Receptor α10/CHRNA10 Blocking Peptide (#BLP-NC010).
Western blot analysis of mouse brain lysates:1. Anti-Nicotinic Acetylcholine Receptor α10 (CHRNA10) Antibody (#ANC-010), (1:400).
2. Anti-Nicotinic Acetylcholine Receptor α10 (CHRNA10) Antibody, preincubated with Nicotinic Acetylcholine Receptor α10/CHRNA10 Blocking Peptide (#BLP-NC010).
Properties
Sequence
- (C)RRHEDWKRLARVMDR, corresponding to amino acid residues 411-425 of rat nAChRα10 (Accession Q9JLB5).
Accession (Uniprot) Number Q9JLB5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
