Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)KELVKNYNPLER, corresponding to amino acid residues 31-42 of rat nAChRα7 (Accession Q05941).
Accession (Uniprot) Number Q05941
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates:1,2. Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007), (1:200).
3,4. Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α7/CHRNA7 (extracellular) Blocking Peptide (#BLP-NC007).- Rat dorsal root ganglion (DRG) frozen sections (1:200).
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Guinea Pig Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007-GP), (1:500).
3-4. Guinea Pig Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007-GP), preincubated with Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Blocking Peptide (#BLP-NC-007).
Last update: 02/01/2024
