Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nicotinic Acetylcholine Receptor α9/CHRNA9 Blocking Peptide (#BLP-NC019) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody (#ANC-019) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α9/CHRNA9 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody (#ANC-019), (1:400).
3,4. Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody, preincubated with Nicotinic Acetylcholine Receptor α9/CHRNA9 Blocking Peptide (#BLP-NC019).
Expression of nAChRα9 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody (#ANC-019), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. nAChRα9 staining (in green) appears in neuronal soma (arrows). DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)KDHKATNSKGSEWKK, corresponding to amino acid residues 436-450 of rat nAChRα9 (Accession P43144).
Accession (Uniprot) Number P43144
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
