Overview
Type: Synthetic peptide
Form: Lyophilized powder
NKCC1/SLC12A2 Blocking Peptide (#BLP-NT071) is the original antigen used for immunization during Anti-NKCC1 (SLC12A2) Antibody (#ANT-071) generation. The blocking peptide binds and ‘blocks’ Anti-NKCC1/SLC12A2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat lung lysate (lanes 3 and 6):1-3. Anti-NKCC1 (SLC12A2) Antibody (#ANT-071), (1:200).
4-6. Anti-NKCC1 (SLC12A2) Antibody, preincubated with NKCC1/SLC12A2 Blocking Peptide (#BLP-NT071).
Expression of NKCC1 in rat cerebellumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NKCC1 (SLC12A2) Antibody (#ANT-071), (1:100). A. NKCC1 staining (red) is detected in the cerebellar molecular layer (Mol). B. DAPI counterstain (blue) reveals the outline of cerebellar layers. C. Merge of the two images.
Properties
Sequence
- (C)RRQAMKEMSIDQAK, corresponding to amino acid residues 828-841 of human NKCC1 (Accession P55011).
Accession (Uniprot) Number P55011
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
