Overview
Type: Synthetic peptide
Form: Lyophilized powder
NKCC2/SLC12A1 Blocking Peptide (#BLP-NT072) is the original antigen used for immunization during Anti-NKCC2 (SLC12A1) Antibody (#ANT-072) generation. The blocking peptide binds and ‘blocks’ Anti-NKCC2/SLC12A1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) kidney membranes:1,2. Anti-NKCC2 (SLC12A1) Antibody (#ANT-072), (1:400).
3,4. Anti-NKCC2 (SLC12A1) Antibody, preincubated with NKCC2/SLC12A1 Blocking Peptide (#BLP-NT072).
Properties
Sequence
- (C)GYKKNWRKAPLSELE, corresponding to amino acid residues 776-790 of rat NKCC2 (Accession P55016).
Accession (Uniprot) Number P55016
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
