Cat #: ESD-750_PACK
Size: 11 Vials
Form: Lyophilized
Antibodies
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-NMDAR2B (GluN2B) (extracellular) Antibody Cat #: AGC-003
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)NTHEKRIYQSNMLNR, corresponding to amino acid residues 323-337 of rat NMDA receptor 2B (Accession Q00960). Extracellular, N-terminus.
Homology
Human, mouse - identical.
Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody Cat #: AGC-003-AR
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH
Reactivity:
H, M, R
Immunogen
Peptide (C)NTHEKRIYQSNMLNR, corresponding to amino acid residues 323-337 of rat NMDAR2B (Accession Q00960). Extracellular, N-terminus.
Homology
Human, mouse - identical.
Label
ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
Pharmacological Reagents
trans-ACBD Cat #: A-280
Size:
10 mg
Source: Synthetic
MW: 159.14
Purity: >95%
Effective concentration
0.1-20 µM.
Activity
trans-ACBD is an NMDA receptor agonist1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
500 mM in H2O. Centrifuge all product preparations before use (10,000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
NMDA Cat #: N-170
Size:
250 mg
Source: Synthetic
MW: 147.1
Purity: >98%
Effective concentration
6.5-500 µM.
Activity
Prototypic NMDA receptor agonist. An amino acid that, as a D-isomer, is the defining agonist for the NMDA receptor subtype of glutamate receptors1. NMDA is a water-soluble synthetic substance that is not normally found in biological tissues2. EC50 ranges between 6.5-25.0 μM, depending on the tissue applied on1,3,4.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Water. Centrifuge all product preparations before use (10000 x g for 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
7-Chlorokynurenic acid sodium salt Cat #: C-286
Size:
25 mg
Source: Synthetic
MW: 245.59
Purity: >98%
Effective concentration
1-100 µM.
Activity
7-Chlorokynurenic acid is an NMDA receptor antagonist that acts at the glycine site with IC50 of 3.4 μM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in H2O. Centrifuge all product preparations before use (10,000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
D-AP5 Cat #: D-145
Size:
25 mg
Source: Synthetic
MW: 197.1
Purity: >95%
Effective concentration
100 nM - 100 µM.
Activity
D-AP5 is an NMDA receptor antagonist. IC50 = 0.28 µM (NR2A), 0.46 µM (NR2B), 1.6 µM (NR2C)1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
25 mg/ml in H2O. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
5,7-Dichlorokynurenic acid Cat #: D-190
Size:
50 mg
Source: Synthetic
MW: 258.06
Purity: >98%
Effective concentration
0.5-20 µM.
Activity
5,7-Dichlorokynurenic acid is an NMDA receptor antagonist acting at the glycine site1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
200 mM in DMSO. Centrifuge all product preparations before use (10 000 X g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
(+)-MK 801 maleate Cat #: M-230
Size:
50 mg
Source: Synthetic
MW: 337.4
Purity: >99% (HPLC)
Effective concentration
0.01-10 µM.
Activity
(+)-MK 801 maleate is a potent, selective and non-competitive NMDA receptor antagonist with an IC50 of 0.01 μM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Up to 100 mM in DMSO. Centrifuge all product preparations before use (5,000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
PEAQX tetrasodium hydrate Cat #: P-255
Size:
10 mg
Source: Synthetic
MW: 560.15
Purity: >95%
Effective concentration
10 nM - 1 µM.
Activity
PEAQX is an NMDA receptor antagonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in water. Centrifuge all product preparations before use (10,000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For more information, please refer to individual certificate of analysis for each product.
For research purposes only, not for human use
