Overview
Type: Synthetic peptide
Form: Lyophilized powder
NMDAR2B/GluN2B (extracellular) Blocking Peptide (#BLP-GC003) is the original antigen used for immunization during Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003) generation. The blocking peptide binds and ‘blocks’ Anti-NMDAR2B/GluN2B (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates:1. Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003), (1:600).
2. Anti-NMDAR2B (GluN2B) (extracellular) Antibody, preincubated with NMDAR2B/GluN2B (extracellular) Blocking Peptide (#BLP-GC003).
Expression of NMDA receptor 2B in rat hippocampusImmunohistochemical staining of rat hippocampal CA1 frozen sections stained with Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003), (1:100). A. NMDAR2B (green) appears in the pyramidal layer of CA1. B. Staining of neurofilament 200 (red) identifies neuronal processes. C. Confocal merge demonstrates localization of NMDAR2B in cells and not in processes.
Expression of NMDA receptor 2B in rat cortexImmunohistochemical staining of rat parietal cortex frozen sections stained with Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003), (1:100). A. NMDAR2B (green) appears in the pyramidal layer of layer 5. B. Staining of neurofilament 200 (red) identifies neuronal processes. C. Confocal merge demonstrates localization of NMDAR2B in cells.
Properties
Sequence
- (C)NTHEKRIYQSNMLNR, corresponding to amino acid residues 323-337 of rat NMDA receptor 2B (Accession Q00960).
Accession (Uniprot) Number Q00960
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
