Overview
Type: Synthetic peptide
Form: Lyophilized powder
NMDAR2C/GRIN2C (extracellular) Blocking Peptide (#BLP-GC018) is the original antigen used for immunization during Anti-NMDAR2C (GRIN2C) (extracellular) Antibody (#AGC-018) generation. The blocking peptide binds and ‘blocks’ Anti-NMDAR2C/GRIN2C (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane:1. Anti-NMDAR2C (GRIN2C) (extracellular) Antibody (#AGC-018), (1:200).
2. Anti-NMDAR2C (GRIN2C) (extracellular) Antibody, preincubated with NMDAR2C/GRIN2C (extracellular) Blocking Peptide (#BLP-GC018).
Western blot analysis of mouse brain lysate:1. Anti-NMDAR2C (GRIN2C) (extracellular) Antibody (#AGC-018), (1:200).
2. Anti-NMDAR2C (GRIN2C) (extracellular) Antibody, preincubated with NMDAR2C/GRIN2C (extracellular) Blocking Peptide (#BLP-GC018).
Expression of NMDAR2C in rat pancreasImmunohistochemical staining rat pancreas paraffin-embedded sections using Anti-NMDAR2C (GRIN2C) (extracellular) Antibody (#AGC-018), (1:100) followed by goat-anti-rabbit-AlexaFluor-594 (red) secondary antibody. Staining is present in endocrine cells of the Isles of Langerhans. Hoechst 33342 is used as the counterstain (blue).
Properties
Sequence
- (C)RHRLWEMVGRWDH, corresponding to amino acid residues 365-377 of rat NMDAR2C (Accession Q00961).
Accession (Uniprot) Number Q00961
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
