Overview
Type: Synthetic peptide
Form: Lyophilized powder
NMDAR2D/GRIN2D (extracellular) Blocking Peptide (#BLP-GC020) is the original antigen used for immunization during Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020) generation. The blocking peptide binds and ‘blocks’ Anti-NMDAR2D/GRIN2D (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020), (1:200).
3,4. Anti-NMDAR2D (GRIN2D) (extracellular) Antibody, preincubated with NMDAR2D/GRIN2D (extracellular) Blocking Peptide (#BLP-GC020).
Expression of NMDA receptor 2D in rat reticular thalamic nucleusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020), (1:200). A. Staining (green) appears in a discreet population of cells (thin arrows). B. The same section was stained for parvalbumin (red) and cell nuclei were visualized with DAPI (blue). Cells positive for NR2D but negative for parvalbumin (thick arrow, shown in A) and cells positive for both NR2D and parvalbumin (vertical arrow) are both present.
Properties
Sequence
- CRTQNRTHRGESLHR, corresponding to amino acid residues 345-359 of rat NMDAR2D (Accession Q62645).
Accession (Uniprot) Number Q62645
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
