Overview
Type: Synthetic peptide
Form: Lyophilized Powder
NMNAT2 Blocking Peptide (#BLP-NR139) is the original antigen used for immunization during Anti-NMNAT2 Antibody (#ANR-139) generation. The blocking peptide binds and ‘blocks’ Anti-NMNAT2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1-2. Anti-NMNAT2 Antibody (#ANR-139), (1:400).
3-4. Anti-NMNAT2 Antibody, preincubated with NMNAT2 Blocking Peptide (#BLP-NR139).
Western blot analysis of human SHSY-5Y neuroblastoma cell lysate:1. Anti-NMNAT2 Antibody (#ANR-139), (1:400).
2. Anti-NMNAT2 Antibody, preincubated with NMNAT2 Blocking Peptide (#BLP-NR139).
Expression of NMNAT2 in mouse fornixImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NMNAT2 Antibody (#ANR-139), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NMNAT2 immunoreactivity (green) appears in the fornix (FX and vertical arrow) and in the hippocampal CA3 region (CA3 and horizontal arrow). B. Pre-incubation of the antibody with NMNAT2 Blocking Peptide (BLP-NR139), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of NMNAT2 in rat fornixImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NMNAT2 Antibody (#ANR-139), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NMNAT2 immunoreactivity (green) appears in the fornix (arrows). B. Pre-incubation of the antibody with NMNAT2 Blocking Peptide (BLP-NR139), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)HENTQPIYQNSNVPTK, corresponding to amino acid residues 131 - 146 of mouse NMNAT2 (Accession Q8BNJ3). Intracellular.
Accession (Uniprot) Number Q8BNJ3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
