Cat #: BLP-NR072
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)KLENASWIHDPLMK, corresponding to amino acid residues 3-16 of rat NMUR2 (Accession Q9ESQ4).
Accession (Uniprot) Number Q9ESQ4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), human brain glioblastoma (U-87 MG) (lanes 3 and 7) and mouse brain glioma (C6) cell (lanes 4 and 8) lysates:1-4. Anti-NMUR2 (extracellular) Antibody (#ANR-072), (1:200).
5-8. Anti-NMUR2 (extracellular) Antibody, preincubated with NMUR2 (extracellular) Blocking Peptide (#BLP-NR072). - Expression of Neuromedin U Receptor 2 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NMUR2 (extracellular) Antibody (#ANR-072), (1:100), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region, showed immunoreactivity (green) in astrocytes (horizontal arrows), and neurons (vertical arrows). B. Pre-incubation of the antibody with NMUR2 (extracellular) Blocking Peptide (BLP-NR072), suppressed staining. Cell nuclei are stained with DAPI (blue).
Last update: 02/01/2024