Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nociceptin Receptor/OPRL1 Blocking Peptide (#BLP-OR015) is the original antigen used for immunization during Anti-Nociceptin Receptor (OPRL1) Antibody (#AOR-015) generation. The blocking peptide binds and ‘blocks’ Anti-Nociceptin Receptor/OPRL1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and U-87 MG (lanes 3 and 6) lysates:1,2. Anti-Nociceptin Receptor (OPRL1) Antibody (#AOR-015), (1:600).
3. Anti-Nociceptin Receptor (OPRL1) Antibody, (1:400).
4-6. Anti-Nociceptin Receptor (OPRL1) Antibody, preincubated with Nociceptin Receptor/OPRL1 Blocking Peptide (#BLP-OR015).
Properties
Sequence
- (C)HREMQVSDRVRSIAKD, corresponding to amino acid residues 337-352 of rat OPRL1 (Accession P35370).
Accession (Uniprot) Number P35370
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
