Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nogo Receptor (extracellular) Blocking Peptide (#BLP-NT008) is the original antigen used for immunization during Anti-Nogo Receptor (extracellular) Antibody (#ANT-008) generation. The blocking peptide binds and ‘blocks’ Anti-Nogo Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-Nogo Receptor (extracellular) Antibody (#ANT-008), (1:200).
2. Anti-Nogo Receptor (extracellular) Antibody, preincubated with Nogo Receptor (extracellular) Blocking Peptide (#BLP-NT008).
Expression of Nogo receptor in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-Nogo Receptor (extracellular) Antibody (#ANT-008). A. NgR (red) appears in Purkinje cells (arrows). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of NgR and calbindin D28K demonstrates some co-localization of these proteins.
Properties
Sequence
- (C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo receptor (Accession Q9BZR6).
Accession (Uniprot) Number Q9BZR6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
