Overview
Type: Synthetic peptide
Form: Lyophilized Powder
NPBWR1 Blocking Peptide (#BLP-NR075) is the original antigen used for immunization during Anti-NPBWR1 Antibody (#ANR-075) generation. The blocking peptide binds and ‘blocks’ Anti-NPBWR1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 4), rat brain lysates (lanes 2 and 5) and rat heart lysate (lanes 3 and 6):1-3. Anti-NPBWR1 Antibody (#ANR-075), (1:400).
4-6. Anti-NPBWR1 Antibody, preincubated with NPBWR1 Blocking Peptide (BLP-NR075).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):1-2. Anti-NPBWR1 Antibody (#ANR-075), (1:400).
3-4. Anti-NPBWR1 Antibody, preincubated with NPBWR1 Blocking Peptide (BLP-NR075).
Expression of NPBWR1 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NPBWR1 Antibody (#ANR-075), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NPBWR1 immunoreactivity (green) appears in the mossy fiber terminal zone (MFT, arrows). B. Pre-incubation of the antibody with NPBWR1 Blocking Peptide (BLP-NR075), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)ESRRVSGRTYGAAR, corresponding to amino acid residues 145 - 158 of mouse NPBWR1 (Accession P49681).
Accession (Uniprot) Number P49681
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
