Overview
Type: Synthetic peptide
Form: Lyophilized powder
NPY1R (extracellular) Blocking Peptide (#BLP-NR027) is the original antigen used for immunization during Anti-NPY1R (extracellular) Antibody (#ANR-027) generation. The blocking peptide binds and ‘blocks’ Anti-NPY1R (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1, 2. Anti-NPY1R (extracellular) Antibody (#ANR-027), (1:200).
3, 4. Anti-NPY1R (extracellular) Antibody, preincubated with NPY1R (extracellular) Blocking Peptide (#BLP-NR027).
Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):1, 2. Anti-NPY1R (extracellular) Antibody (#ANR-027), (1:200).
3, 4. Anti-NPY1R (extracellular) Antibody, preincubated with NPY1R (extracellular) Blocking Peptide (#BLP-NR027).
Expression of NPY1R in rat striatum and subventricular zone (SVZ)Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NPY1R (extracellular) Antibody (#ANR-027), (1:200), followed by goat anti-rabbit-AlexaFluor-488. NPY1R immunoreactivity (green) appears in the striatum (STR) and the SVZ. Cell nuclei were stained with DAPI (blue). LV = Lateral ventricle.
Properties
Sequence
- CFDKFPSDSHRLSY, corresponding to amino acid residues 197-210 of mouse NPY1R (Accession Q04573).
Accession (Uniprot) Number Q04573
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
