Overview
Type: Synthetic peptide
Form: Lyophilized powder
NPY4R Blocking Peptide (#BLP-NR024) is the original antigen used for immunization during Anti-NPY4R Antibody (#ANR-024) generation. The blocking peptide binds and ‘blocks’ Anti-NPY4R primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (lanes 1 and 4), cortex (lanes 2 and 5), and hippocampus (lanes 3 and 6) lysates:1,2,3. Anti-NPY4R Antibody (#ANR-024), (1:200).
4,5,6. Anti-NPY4R Antibody, preincubated with NPY4R Blocking Peptide (#BLP-NR024).- Rat retinal sections (1:200) (Martins, J. et al. (2015) ASN. Neuro. 7, 4.).
Properties
Sequence
- NINFKKDIKALVLTC corresponding to amino acid residues 326-340 of rat NPY4R (Accession Q63447).
Accession (Uniprot) Number Q63447
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
