Overview
Type: Synthetic peptide
Form: Lyophilized powder
NRG1 Type III (extracellular) Blocking Peptide (#BLP-NR113) is the original antigen used for immunization during Anti-NRG1 Type III (extracellular) Antibody (#ANR-113) generation. The blocking peptide binds and ‘blocks’ Anti-NRG1 Type III (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat dorsal root ganglion lysate:1. Anti-NRG1 Type III (extracellular) Antibody (#ANR-113), (1:200).
2. Anti-NRG1 Type III (extracellular) Antibody, preincubated with NRG1 Type III (extracellular) Blocking Peptide (#BLP-NR113).
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1,2. Anti-NRG1 Type III (extracellular) Antibody (#ANR-113), (1:200).
3,4. Anti-NRG1 Type III (extracellular) Antibody, preincubated with NRG1 Type III (extracellular) Blocking Peptide (#BLP-NR113).- Mouse brain sections (Gatius, A. et al. (2020) Front. Cell. Neurosci. 13, 582.).
Properties
Sequence
- (C)DKIFEYDSPTHLD, corresponding to amino acid residues 101-113 of human Neuregulin-1 Type III (Accession Q02297).
Accession (Uniprot) Number Q02297
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
