Overview
Type: Synthetic peptide
Form: Lyophilized powder
NRG2 (extracellular) Blocking Peptide (#BLP-NR114) is the original antigen used for immunization during Anti-NRG2 (extracellular) Antibody (#ANR-114) generation. The blocking peptide binds and ‘blocks’ Anti-NRG2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1,2. Anti-NRG2 (extracellular) Antibody (#ANR-114), (1:200).
3,4. Anti-NRG2 (extracellular) Antibody, preincubated with NRG2 (extracellular) Blocking Peptide (#BLP-NR114).
Expression of Neuregulin-2 in mouse hippocampus and cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NRG2 (extracellular) Antibody (#ANR-114), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NRG2 staining in CA1 hippocampal region (green) is detected in the pyramidal layer (P, arrows). B. Staining in the cerebellum (green) is observed in Purkinje cells (arrows). Mol= Molecular layer; GL= Granular layer. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RDIRIKYGNGRKNSR, corresponding to amino acid residues 297-311 of rat Neuregulin-2 (Accession O35569).
Accession (Uniprot) Number O35569
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
