Overview
Cat #:
STC-180
Alternative Name Bu8, Omega-conotoxin Bu8
Lyophilized Powder yes
Origin Conus bullatus (Bubble cone)
Source Synthetic peptide
MW: 2753 Da
Purity: >98%
Form Lyophilized Powder
Effective concentration 10 – 100 nM
Sequence CKRKGSSCRRTSYDCCTGSCRNGKC – NH2
Modifications Disulfide bonds location- Cys1-Cys16, Cys8-Cys20, Cys15-Cys25
Cys25 – C-terminal amidation
Molecular formula C103H174N42O35S6
Activity Specific and potent N-type voltage-gated calcium (CaV2.2) channel blocker1.
Shipping and storage The product is shipped as a lyophilized powder at room temperature. Upon receipt, it should be stored at -20°C. Protect from moisture.
Solubility Soluble in water. For long-term storage in solution, we recommend to prepare a stock solution by dissolving the product in ddH2O at a concentration X100-1000 of final working solution. Divide the solution into small aliquots and store at -20°C. Upon use, thaw the relevant vial intended for use and dilute in your desired working buffer. The preparation of fresh solutions in working buffers before use is recommended. Repeat freeze-thawing might result in loss of activity. Centrifuge all product preparations before use (10000 x g, 5 min).
Storage of solutions The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the toxin in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Scientific background ω-Conotoxin Bu8 (Bu8) is a 25 amino acid peptidyl toxin that was synthesized from the venom of the cone snail, Conus bullatus1. This toxin potently and selectively blocks N-type voltage-gated calcium (Cav2.2) channels. Bu8 displays higher or similar analgesic activity in the pain models compared to MVIIA but has less adverse effects1.
Bu8 adopts a canonical globular scaffold, demonstrated as an inhibitory cysteine knot motif, marked by 3 disulfide bonds that is consistent for all ω-conotoxins in the all-crossing pattern of 1-4, 2-5, and 3-61.
Cav2.2 are predominantly expressed in nerve terminals and are involved in the regulation of neuronal excitability and nociceptive signaling. These channels are multifunctional and play important roles in the transduction of acute and chronic pain perception. They are able to transduce electrical activity into other cellular functions, regulate calcium homeostasis, and process pain information. Since ω-conotoxins selectively inhibit Cav2.2 in nociceptors, they are considered attractive molecules for drug design2.
Target CaV2.2 Ca2+ channel
Peptide Content: 100%
Lyophilized Powder
For research purposes only, not for human use
Last Update: 07/05/2024
